Neutral sphingomyelinase-3 mediates TNF-stimulated oxidant activity in skeletal muscle

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Neutral sphingomyelinase-3 mediates TNF-stimulated oxidant activity in skeletal muscle

AIMS Sphingolipid and oxidant signaling affect glucose uptake, atrophy, and force production of skeletal muscle similarly and both are stimulated by tumor necrosis factor (TNF), suggesting a connection between systems. Sphingolipid signaling is initiated by neutral sphingomyelinase (nSMase), a family of agonist-activated effector enzymes. Northern blot analyses suggest that nSMase3 may be a str...

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Sphingomyelinase promotes oxidant production and skeletal muscle contractile dysfunction through activation of NADPH oxidase

Elevated concentrations of sphingomyelinase (SMase) have been detected in a variety of diseases. SMase has been shown to increase muscle derived oxidants and decrease skeletal muscle force; however, the sub-cellular site of oxidant production has not been elucidated. Using redox sensitive biosensors targeted to the mitochondria and NADPH oxidase (Nox2), we demonstrate that SMase increased Nox2-...

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Sphingomyelinase stimulates oxidant signaling to weaken skeletal muscle and promote fatigue.

Sphingomyelinase (SMase) hydrolyzes membrane sphingomyelin into ceramide, which increases oxidants in nonmuscle cells. Serum SMase activity is elevated in sepsis and heart failure, conditions where muscle oxidants are increased, maximal muscle force is diminished, and fatigue is accelerated. We tested the hypotheses that exogenous SMase and accumulation of ceramide in muscle increases oxidants ...

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Neutral proteinase activity in skeletal muscle from thermally injured rats.

Male Sprague-Dawley rats that received 60% total body surface, full-thickness, scald burns on the dorsum and abdomen were used in this study. Neutral proteinase and Ca2+-activated neutral proteinase activities were measured in gastrocnemius and soleus muscles at 3 and 21 days after the thermal injury. Neutral proteinase activity decreased significantly in the soleus (50%) and gastrocnemius (46%...

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Neutral sphingomyelinase 2 is required for cytokine-induced skeletal muscle calpain activation.

Calpain contributes to infection-induced diaphragm dysfunction but the upstream mechanism(s) responsible for calpain activation are poorly understood. It is known, however, that cytokines activate neutral sphingomyelinase (nSMase) and nSMase has downstream effects with the potential to increase calpain activity. We tested the hypothesis that infection-induced skeletal muscle calpain activation ...

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ژورنال

عنوان ژورنال: Redox Biology

سال: 2014

ISSN: 2213-2317

DOI: 10.1016/j.redox.2014.07.006